lecture notes-enzyme-2-Enzyme kinetics

lecture notes-enzyme-2-Enzyme kinetics - Enzyme Kinetics...

Info iconThis preview shows pages 1–9. Sign up to view the full content.

View Full Document Right Arrow Icon
Enzyme Kinetics: Study the rate of enzyme catalyzed reactions. - Models for enzyme kinetics - Michaelis-Menten kinetics - Inhibition kinetics - Effect of pH and Temperature Enzyme Kinetics
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Enzyme Kinetics Michaelis-Menten kinetics or saturation kinetics which was first developed by V.C.R. Henri in 1902 and developed by L. Michaelis and M.L. Menten in 1913. This model is based on data from batch reactors with constant liquid volume. - Initial substrate, [S 0 ] and enzyme [E 0 ] concentrations are known. - An enzyme solution has a fixed number of active sites to which substrate can bind. - At high substrate concentrations, all these sites may be occupied by substrates or the enzyme is saturated.
Background image of page 2
Saturation Enzyme Kinetics
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
M-M Enzyme Kinetics Saturation kinetics can be obtained from a simple reaction scheme that involves a reversible step for enzyme-substrate complex formation and a dissociation step of the ES complex. ] [ ] [ 2 ES k dt P d v = = K1 E P k ES + → 2 E+S K-1 where the rate of product formation v (moles/l-s, g/l- K i is the respective reaction rate constant.
Background image of page 4
Enzyme Kinetics The rate of variation of ES complex is Since the enzyme is not consumed, the conservation equation on the enzyme yields ] [ 2 ] [ 1 ] ][ [ 1 ] [ ES k ES k S E k dt ES d - - - = ] [ ] 0 [ ] [ ES E E - =
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Enzyme Kinetics ] [ 2 ] [ 1 ] ][ [ 1 ] [ ES k ES k S E k dt ES d - - - = ] [ ] 0 [ ] [ ES E E - = ] [ ] [ 2 ES k dt P d v = = How to use independent variable [S] to represent v?
Background image of page 6
At this point, an assumption is required to achieve an analytical solution. - The rapid equilibrium assumption Michaelis - Menten Approach. - The quasi-steady-state assumption. Briggs and Haldane Approach. Enzyme Kinetics
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
The rapid equilibrium assumption: - Assumes a rapid equilibrium between the enzyme and substrate to form an [ES] complex. E
Background image of page 8
Image of page 9
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 29

lecture notes-enzyme-2-Enzyme kinetics - Enzyme Kinetics...

This preview shows document pages 1 - 9. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online