lecture notes-enzyme-3-Inhibition kinetics

lecture notes-enzyme-3-Inhibition kinetics - Inhibited...

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Inhibited Enzyme Kinetics Inhibitors may bind to enzyme and reduce their activity. Enzyme inhibition may be reversible or irreversible. For reversible enzyme inhibition, there are - competitive - noncompetitive - uncompetitive
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Inhibited Enzyme Kinetics Competitive inhibitors (I) - substrate analogs - compete with substrate for the active site of the enzyme K1 E P k ES + → 2 E+S K-1 + I EI K I
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Inhibited Enzyme Kinetics Competitive inhibitors (I) Assume rapid equilibrium and with the definitions of ] [ ] [ ] [ ] 0 [ EI ES E E + + = ] [ ] [ 2 ES k dt P d v = = ] [ ] ][ [ 1 1 ' ES S E k k m K = - = ] [ ] ][ [ EI I E I K =
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Inhibited Enzyme Kinetics Competitive inhibitors (I), we can obtain, ] [ ) / ] [ 1 ( ' ] [ S I K I m K S m V v + + = ] [ ' , ] [ S app m K S m V v + = ) / ] [ 1 ( ' ' , I K I m K app m K + = When [I] =0, ' ' , m K app m K = m V remains same as that in Michaelis-Menten equation. app m K , is increased.
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Inhibited Enzyme Kinetics Noncompetitive inhibitors (I) - not substrate analogs - bind on sites other than the active sites E P k ES + → 2 E+S + I EI+S K I + I ESI K I Km’ Km’
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lecture notes-enzyme-3-Inhibition kinetics - Inhibited...

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