Lecture notes-enzyme-Diffusional Limitation in Immobilized Enzyme System

Lecture notes-enzyme-Diffusional Limitation in Immobilized Enzyme System

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Diffusional Limitation in Immobilized Enzyme Systems Immobilized enzyme systems normally include - insoluble immobilized enzyme - soluble substrate, or product They are heterogeneous systems
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CONCENTRATION DIFFERENCE FILM TRANSFER DRIVING FORCE DIFFUSION HIGH Immobilized Enzyme S b ELECTRIC ATTRACTION Low S concentration Substrate
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CONCENTRATION DIFFERENCE FILM TRANSFER DRIVING FORCE DIFFUSION HIGH Immobilized Enzyme S b ELECTRIC ATTRACTION REACTION PRODUCT
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FILM TRANSFER DRIVING FORCE DIFFUSION HIGH Immobilized Enzyme S b INTRA-PARTICLE TRANSFER
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FILM TRANSFER PRODUCT HIGH Immobilized Enzyme S b IN TRA-PARTICLE TRANSFER REACTION
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Diffusional Limitation in Immobilized Enzyme Systems In immobilized enzyme systems, the overall production rate is determined by - liquid film mass transfer (external diffusion) substrate, product - intraparticle mass transfer (internal diffusion) substrate, product in porous supports - enzyme catalysis reaction
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Diffusional Limitation in Immobilized Enzyme Systems Ss: substrate concentration at the surface; Sb: substrate concentration in bulk solution. Enzyme Ss Sb Liquid Film Thickness, L E P k ES + → 2 E+S Diffusion Effects in Surface-bound Enzymes on Nonporous Support Materials Assume the enzyme catalyzed reaction rate follows Michaelis-Menten type kinetics.
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Enzyme Ss Sb Liquid Film Thickness, L No intraparticle diffusion Assume: - Enzymes are evenly distributed on the surface of a nonporous support material.
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