Lecture notes-enzyme-Diffusional Limitation in Immobilized Enzyme System

Lecture notes-enzyme-Diffusional Limitation in Immobilized Enzyme System

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Unformatted text preview: Diffusional Limitation in Immobilized Enzyme Systems • Immobilized enzyme systems normally include- insoluble immobilized enzyme- soluble substrate, or product • They are heterogeneous systems C O N C E N T R A T I O N D I F F E R E N C E F I L M T R A N S F E R DRIVING FORCE DIFFUSION HIGH Immobilized Enzyme S b E L E C T R I C A T T R A C T I O N Low S concentration Substrate C O N C E N T R A T I O N D I F F E R E N C E F I L M T R A N S F E R DRIVING FORCE DIFFUSION HIGH Immobilized Enzyme S b E L E C T R I C A T T R A C T I O N REACTION PRODUCT F I L M T R A N S F E R DRIVING FORCE DIFFUSION HIGH Immobilized Enzyme S b I N T R A- P A R T I C L E T R A N S F E R F I L M T R A N S F E R PRODUCT HIGH Immobilized Enzyme S b I N T R A- P A R T I C L E T R A N S F E R REACTION Diffusional Limitation in Immobilized Enzyme Systems In immobilized enzyme systems, the overall production rate is determined by- liquid film mass transfer (external diffusion) substrate, product- intraparticle mass transfer (internal diffusion) substrate, product in porous supports - enzyme catalysis reaction Diffusional Limitation in Immobilized Enzyme Systems Ss: substrate concentration at the surface; Sb: substrate concentration in bulk solution. Enzyme Ss Sb Liquid Film Thickness, L E P k ES + → 2 E+S Diffusion Effects in Surface-bound Enzymes on Nonporous Support Materials Assume the enzyme catalyzed reaction rate follows Michaelis-Menten type kinetics. Enzyme Ss Sb Liquid Film Thickness, L No intraparticle diffusion Assume:- Enzymes are evenly distributed on the surface of a nonporous support material.material....
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This note was uploaded on 02/13/2012 for the course CHM 3210 taught by Professor Kennell during the Fall '11 term at University of Florida.

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Lecture notes-enzyme-Diffusional Limitation in Immobilized Enzyme System

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