lecture notes-growth kinetics--growth in continous culture

lecture notes-growth kinetics--growth in continous culture...

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Unformatted text preview: Cells Growth in Continuous Culture Continuous culture: fresh nutrient medium is continually supplied to a well-stirred culture and products and cells are simultaneously withdrawn. At steady state, concentrations of cells, products and substrates are constant . In batch culture: the culture environment changes continually. growth, product formation and substrate utilization terminate after a certain time interval. A continuous-culture laboratory setup medium Products, cells Ideal Chemostat Same as perfectly mixed continuous-flow, stirred-tank reactor (CFSTR).- Control elements: pH, dissolved oxygen, temperature- Fresh sterile medium is fed to the completely mixed and aerated (if required) reactor.- Suspension is removed at the same rate.- Liquid volume in the reactor is kept constant. Cell Growth in Ideal Chemostat A material balance on the cell and substrate concentration around the chemostat yields, dt dX R V X d k R V X g R V FX FX =- +- F is the volumetric flowrate of nutrient solution (l/h); V R is the culture volume (l) (constant); X is the cell concentration (g/l); P is the extracellular product (g/l); g and k d are growth rate and endogenous rate constant, respectively (h-1 ). Subscript 0 denotes the parameters at the feed medium. d t d S R V s p Y X p q R V Y X g R V F S F S M S X =--- / 1 1 / q p is the specific rate of extracellular product formation (g P/g cells-h) Y p/s is the product yield coefficient (g P/g S). Cell Growth in Ideal Chemostat...
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lecture notes-growth kinetics--growth in continous culture...

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