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Lecture - Basics of Molecular Biology Central Dogma DNA replication Transcription Translation Metabolic regulation Genetic level Metabolic pathway

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Unformatted text preview: Basics of Molecular Biology Central Dogma:- DNA replication- Transcription- Translation Metabolic regulation:- Genetic level- Metabolic pathway control Central Dogma Central Dogma: universal- is stored on the DNA molecule.- : The genetic information can be replicated directly to form a second identical molecule.- : Segments information on the DNA molecule can be transcribed to yield m-, t-, r-RNAs.- : Using RNAs, this information is translated into proteins. Cell Division Cycle (Mitosis) m i t o t i c ( M ) p h a s e DNA Replication in Transcription Transcription requires: RNA polymerases- Growth of RNA polymers is energy requiring. e.g. ATP, GTP, The base-sequence of RNA is the precise complement . DNA template RNA product A U T A G C C G The primary product of transcription: m-RNA, t-RNA and r-RNA. m-RNA RNA polymerase Promoter Gene 1 Gene 2 Gene 3 Transcription DNA template http://www-class.unl.edu/biochem/gp2/m_biology/animation/gene/gene_a2.html Operator Transcription Process- Initiation: The sigma factor of RNA polymerase recognizes a specific sequence of nucleotides on a DNA strand – promoter region. the strands unwound.- Elongation: Transcription starts with the core enzyme of the RNA polymerase then the sigma factor is released....
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This note was uploaded on 02/13/2012 for the course CHM 3210 taught by Professor Kennell during the Fall '11 term at University of Florida.

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Lecture - Basics of Molecular Biology Central Dogma DNA replication Transcription Translation Metabolic regulation Genetic level Metabolic pathway

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