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lecture notes-separation and purification-1-separation of solid from liquid-web

Lecture notes-separation and purification-1-separation of solid from liquid-web

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Unformatted text preview: Recovery and Purification of Bio-Products (Chapter 11, M. Shular, textbook)- Strategies to recovery and purify bio- products- Solid-liquid separation- Cell disruption- Separation of soluble products- Finishing steps for purification Recovery and Purification of Bio-Products- Strategies to recovery and purify bio-products Unique characteristics of bioseparation products:- the products are in concentration in an aqueous medium. e.g therapeutic protein 0.01mg/l.- The products are usually sensitive.- There is a of products to be separated.- The products can be , often as insoluble inclusion bodies.- The physical and chemical properties of products are similar to contaminants.- Extremely purity and homogeneity may be needed for human care. Recovery and Purification of Bio-Products- Strategies to recovery and purify bio-products Fementer Solid-liquid separation Recovery Purification Supernatant Cells Cell products Cell rupture Cell debris Crystallization and drying Recovery and Purification of Bio-Products- Strategies to recovery and purify bio-products Fementer Solid-liquid separation Recovery Purification Supernatant Cells Cell products Cell rupture Cell debris Crystallization and drying Recovery and Purification of Bio-Products- Liquid and solid separation- solid particles: mainly cellular mass, specific gravity 1.05-1.1. size (diameter): bacterial cells: 0.5-3 µm yeast cells: 5 -10 µm mold: 5-15 µm in diameter and 50-500 µm in length animal cells: 10 µm plant cells: 20 µm Methods: . Liquid-Solid Separation...
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Lecture notes-separation and purification-1-separation of solid from liquid-web

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