lecture notes-separation and purification-2-cell disruption-separation of soluble products-web

Lecture notes-separation and purification-2-cell disruption-separation of soluble products-web

Info iconThis preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Recovery and Purification of Bio-Products- Strategies to recovery and purify bio-products Fermenter Solid-liquid separation Recovery Purification Supernatant Cells Cell products Cell disruption or rupture Cell debris Crystallization and drying Cell Disruption Disruption: the cell envelope is physically broken, releasing all intracellular components into the surrounding medium Methods: Mechanical and non mechanical - Mechanical- (sonicators) bacteria, virus and spores suspensions at lab-scale Electronic generatorultrasonic waves mechanical oscillation by a titanium probe immersed in a cell disruption. http://www.biologics-inc.com/sd-models.htm Cell Disruption- Mechanical : continuous operation, Algae, bacteria and fungi Large scale, up to 2000kg/h liquid and solid Principle of operation: A grinding chamber filled with about 80% beads. A shaft with designed discs or impellers is within the chamber. The shift rotates at high speeds, high shearing and impact forces from the beads break the cell wall. http://www.cbmills.com/Products/horizontalmills.htm Dyno-Mill (liquid) Cell Disruption- Mechanical Ball Mill : solid Frozen cell paste, cells attached to or within a solid matrix. Large scale http://www.unitednuclear.com/mills.htm Cell Disruption- Mechanical : suspension, large scale To pump a slurry (up to 1500 bar) through a restricted orifice valve. The cells disrupt as they are extruded through the valve to atmosphere pressure by- high liquid shear in the orifice - sudden pressure drop upon discharge i.e. French press, Gaulin-Manton, Rannie high-pressure homogenizer High pressure orifice Cell Disruption- Nonmechanical- : use chemicals to solubilise the components in the cell walls to release the product. Chemical requirements:- products are insensitive to the used chemicals.- the chemicals must be easily separable. Types of chemicals:- surfactants (solubilising lipids): sodium sulfonate, sodium dodecylsulfate.- Alkali: sodium hydroxide, harsh- Organic solvents: penetrating the lipids and swelling the cells. e.g. toluene....
View Full Document

Page1 / 26

Lecture notes-separation and purification-2-cell disruption-separation of soluble products-web

This preview shows document pages 1 - 7. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online