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MicroControlPt1121_OLS - Controlling Microbial Growth Part...

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Controlling Microbial Growth Part 1: Use of Physical and Chemical Methods Physical and chemical methods are used to control microbial growth on inanimate items and body surfaces Sterilization Destruction of all forms of microbial life, even endospores Disinfection Destruction of pathogenic microbes (not endospores) on inanimate objects Sanitization Cleaning process intended to lower number of microbes on plates, glasses, etc. to safe public health levels Antisepsis Kill or inhibit growth of microbes (not endospores) on body surfaces Biocidal methods : Kill microbes Microbial death is defined as the permanent loss of the ability to reproduce Biostatic methods : Inhibit growth (if treatment is stopped microbes may begin reproducing again) Microbes highly resistant to physical & chemical methods Prions, Endospores Microbes moderately resistant to physical & chemical methods Protozoan cysts, Nonenveloped viruses, Certain bacteria ( Mycobacterium tuberculosis, Staph. aureus, Pseudomonas ) Microbes least resistant to physical & chemical methods Most bacteria, Fungi, Fungal spores, Protozoa, Enveloped viruses Factors that influence the effectiveness of physical & chemical methods: 1
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1. Number of microbes present 2. Types of microbes present 3. Time of treatment 4. Concentration of chemical or intensity of physical treatment 5. Treatment Environment A.
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