Biol302 HW Spring2011

Biol302 HW Spring2011 - BglII at 5’ end and PstI at...

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Homework for Biol302 due in class May 18 th : Codon Optimization Website: http://www.encorbio.com/protocols/Codon.htm Format as follows: Paul Feinstein Biol302 April 13 th , 2010 Worked with: Part I (20pts) Multi-exonic human gene codon optimized to bacteria with Restriction Sites EcoRI at 5’ end and BamHI at 3’ end. a) Name and Starting amino acid sequence of gene used: b) Codon optimized nucleotide sequence of human gene with restriction sites: c) Design amplification oligos for codon optimized gene: Oligo 1: Oligo 2: Part II (20pts) Bacterial gene codon optimized to mammals with Restriction Sites
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Unformatted text preview: BglII at 5’ end and PstI at 3’end. a) Name and Starting amino acid sequence of gene used: b) Codon optimized nucleotide sequence of bacteria gene with restriction sites: Be sure to add Kozak sequence GCCACC ATG . Be sure to alter codon usage such that there are no polyA addition sites of AATAAA . Be sure to alter codon usage such that there are no splice donor or acceptor sites. Splice donor sequence to avoid is: GTAAG . Splice acceptor sequence to avoid is: TTTCAG . c) Design amplification oligos for codon optimized gene: Oligo 1: Oligo 2:...
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This note was uploaded on 02/14/2012 for the course BIO 302 taught by Professor Feinstein during the Spring '11 term at Iowa State.

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