Lecture 13 Biol302 Spring 2011

Lecture 13 Biol302 Spring 2011 -...

Info iconThis preview shows pages 1–30. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Transformation-Griffith’s Expt 1928 DNA Mediates Transformation Convert IIR to IIIS By DNA? Avery MacLeod and McCarty Experiment Circa 1943 Transforming Principle + means that activity is present DNAse activity All RNA gets degraded during enzyme preparation A-DNA, B-DNA and Z-DNA The Z-DNA helix is left-handed and has a structure that repeats every 2 base pairs. The major and minor grooves, unlike A- and B-DNA, show little difference in width Non-B DNA in disease Chapter 10 Replication of DNA and Chromosomes DNA Replication is Semiconservative Each strand serves as a template Complementary base pairing determines the sequence of the new strand Each strand of the parental helix is conserved Possible Modes of DNA Replication The Meselson-Stahl Experiment: DNA Replication in E. coli is Semiconservative Visualization of Replication in E. coli Replication in E. coli The Origin of Replication in E. coli Note: OriC is 245bp The Core Origin of Replication in SV 40 Prepriming at oriC in E. coli DNA Polymerases and DNA Synthesis In Vitro Requirements of DNA Polymerases Primer DNA with free 3'-OH Template DNA to specify the sequence of the new strand Substrates: dNTPs Mg 2+ ( where?) Nucleophilic attack of alpha phosphate which releases pyrophosphate β α γ Mg 2+ ( where?) α β γ DNA Polymerase I: 5 ' 3 ' Polymerase Activity Often called: Kornberg Polymerase DNA Polymerase I: 5 ' 3 ' Exonuclease Activity Cleaves ahead of itself DNA Polymerase I: 3 ' 5 ' Exonuclease Activity Proofreading Klenow fragment…..is? DNA Polymerases...
View Full Document

This note was uploaded on 02/14/2012 for the course BIO 302 taught by Professor Feinstein during the Spring '11 term at Iowa State.

Page1 / 69

Lecture 13 Biol302 Spring 2011 -...

This preview shows document pages 1 - 30. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online