Lecture 14 Biol302 Spring 2011

Lecture 14 Biol302 Spring 2011 - Typical Plasmid Blue/White...

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Typical Plasmid
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Blue/White Selection
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Alpha complementation Trick alpha omega
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Blue/White Selection
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Standard 4 exon gene -Where is the polyA tail in the gene above? -Draw a 4 exon gene where exon 4 is all 3’UTR
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Chapter 11 Transcription and RNA Processing
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The Central Dogma
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The Central Dogma
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The Central Dogma
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Transcription and Translation in Prokaryotes The primary transcript is equivalent to the mRNA molecule . The mRNA codons on the mRNA are translated into an amino acid sequence by the ribosomes.
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Transcription and Translation in Eukaryotes The primary transcript (pre-mRNA) is a precursor to the mRNA . The pre-mRNA is modified at both ends, and introns are removed to produce the mRNA. After processing, the mRNA is exported to the cytoplasm for translation by ribosomes.
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Types of RNA Molecules Messenger RNAs (mRNAs) —intermediates that carry genetic information from DNA to the ribosomes. Transfer RNAs (tRNAs) —adaptors between amino acids and the codons in mRNA. Ribosomal RNAs (rRNAs) —structural and catalytic components of ribosomes. Small nuclear RNAs (snRNAs) —structural components of spliceosomes. Micro RNAs (miRNAs) —short single-stranded RNAs that block expression of complementary mRNAs.
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in Eukaryotes Method: Pulse- Chase Labeling At first, labeled RNA is exclusively in the nucleus . Later, the labeled
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This note was uploaded on 02/14/2012 for the course BIO 302 taught by Professor Feinstein during the Spring '11 term at Iowa State.

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Lecture 14 Biol302 Spring 2011 - Typical Plasmid Blue/White...

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