Biol710 Homework 10pts DRAFT

Biol710 Homework 10pts DRAFT - other tissue with that...

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Biol710 Homework November 8 th 2010 Due December 8 th in class or at my office (904N) by 8pm. Please turn in a hard copy (no e-files). 10pts (2pts/experiment) Please describe a series of five independent experiments using the lecture material as a guide. (Each experiment can build on the previous one, but it is not necessary.) Maximum 1 page text plus maximum 1 page figure for each experiment (2 pages total). (Each experiment will be graded as all or none.) Each experiment is meant to teach you something profound about the molecule described below (paragraph 1 or 2 or 3). You may choose ONLY one of the following topics. 1) You have just purified a protein from the pancreas. You have the amino acid sequence and an antibody that can detect it. You cannot detect the protein in any
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Unformatted text preview: other tissue with that antibody by western blotting. You are not sure what it does. It could be a transcription factor, secreted molecule or anything. You think it might be involved in regulating sugar metabolism. 2) You have just cloned a cDNA from Drosophila. It is expressed only in the eye. You think that this gene is used for setting up eye formation and perhaps may do this in any organism. 3) You have just determined that one of your patients has a mutation in a single gene that makes the patient unable to be infected by HIV. It is known that HIV needs at least the T4 receptor to infect immune cells. This new mutation (not in T4) may lay in a “co-receptor”. The speculation is that HIV infection cannot occur when either T4 or the co-receptor are missing....
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This note was uploaded on 02/14/2012 for the course BIO 710L taught by Professor Goldfarb during the Fall '10 term at Iowa State.

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