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Unformatted text preview: 17) Name two ways to make mutations in plasmid. 18) What is the yeast two-hybrid system? 19) What is the one-hybrid system? 20) Name the protein that binds to the TetO sequence? 21) What is GFP? 22) What is Cherry (protein)? 23) What is Venus (protein) ? 24) What is Cerulean (protein) ? 25) What are molecular beacons? 26) Define I.R.E.S. 27) What does the T2A fragment do? 28) What is Translational Frameshifting? 29) What are CRE and Flp? 30) Cre works with LoxP or FRT? Flp works with LoxP or FRT? 31) What is a Bacterial artificial chromosome? 32) How are Transgenic animals made (in general)? Be brief and specific. 33) What are Zn finger nucleases and what are they good for? 34) What do Double Strand DNA breaks promote? 35) What are ES cells? 36) How were mouse ES cells derived? 37) Why do we use ES cells to make Gene-targeted mice? 38) What is meant by Structure/Function analysis?...
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This note was uploaded on 02/14/2012 for the course BIO 710L taught by Professor Goldfarb during the Fall '10 term at Iowa State.
- Fall '10
- molecular biology