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Lecture 4 TEST - 17 Name two ways to make mutations in...

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Name 710.LC GRADUATE MOLECULAR BIOLOGY 9/15/2010 Lecture 4 Competency Test. 1) Name the five components of a PCR reaction. 2) Name two ways to synthesize a gene. 3) What is the purpose of codon optimizing genes? 4) What are the 3 common components of plasmids used in DNA cloning? 5) What is the difference between an oligonucleotide and a primer? 6) Are oligonucleotides and primers single stranded? 7) Do oligonucleotides and primers have to be DNA? 8) Name 4 parameters that affect annealing of two single stranded DNA chains? 9) What does DNA ligase do? 10) What does a Kinase do? 11) What are Restriction Enzymes? 12) Given one 4-cutter restriction enzyme, how many times might it cut a 1000bp dsDNA molecule? 13) Given one 6-cutter restriction enzyme, how many times might it cut a 1000bp dsDNA molecule? 14) What is the most common type of DNA sequencing? 15) What is NextGen Sequencing? 16) What is a transcriptome?
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Unformatted text preview: 17) Name two ways to make mutations in plasmid. 18) What is the yeast two-hybrid system? 19) What is the one-hybrid system? 20) Name the protein that binds to the TetO sequence? 21) What is GFP? 22) What is Cherry (protein)? 23) What is Venus (protein) ? 24) What is Cerulean (protein) ? 25) What are molecular beacons? 26) Define I.R.E.S. 27) What does the T2A fragment do? 28) What is Translational Frameshifting? 29) What are CRE and Flp? 30) Cre works with LoxP or FRT? Flp works with LoxP or FRT? 31) What is a Bacterial artificial chromosome? 32) How are Transgenic animals made (in general)? Be brief and specific. 33) What are Zn finger nucleases and what are they good for? 34) What do Double Strand DNA breaks promote? 35) What are ES cells? 36) How were mouse ES cells derived? 37) Why do we use ES cells to make Gene-targeted mice? 38) What is meant by Structure/Function analysis?...
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