Lecture 6-LC710 1st hr

Lecture 6-LC710 1st hr - Making Human (eukaryote) proteins...

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Making Human (eukaryote) proteins in Bacteria (prokaryote)
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Nucleus
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Mitochondria What keeps the mitochondria in dividing cells?…most likely
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What keeps plasmids in dividing cells? Resistance gene
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Tetracycline works by:
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Tetracycline resistance works by:
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What allows for DNA replication in plasmid? Resistance gene Origin of Replication
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What allows for mRNA production? Resistance gene Origin of Replication Promoter
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MUST Know
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Now have an inducible system Resistance gene Origin of Replication Promoter Add gene
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How does gene need to be formulated For transcription to produce functional RNA occur in prokaryotes? Resistance gene Origin of Replication Promoter Add gene
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KozaK? Kozak sequence: gccaccATGg
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Finding first AUG in Prokaryotes. Shine-Delgarno sequence!
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Resistance gene Origin of Replication Promoter Add gene So we add a Shine-Delgarno seq
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Resistance gene Origin of Replication Promoter gene We missing something? Shine-Delgarno Sigma Termination sequence
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Transcription Prokaryotes
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Resistance gene Origin of Replication Promoter gene What should gene look like? Shine-Delgarno Sigma Termination sequence
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Origin of Replication Promoter gene What should gene look like? Shine-Delgarno
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This note was uploaded on 02/14/2012 for the course BIO 710L taught by Professor Goldfarb during the Fall '10 term at Iowa State.

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Lecture 6-LC710 1st hr - Making Human (eukaryote) proteins...

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