Chapter 20 - DNA Technology Notes

Chapter 20 - DNA Technology Notes - 20 DNA TECHNOLOGY...

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20- DNA TECHNOLOGY GENETIC ENGINEERING: manipulating  genes  and  genom e s RECOMBINANT DNA Combining  DNA from  different  organisms APPLICATIONS OF DNA TECHNOLOGY DIAGNOSIS  OF  DISEASE       Virus  detection;         ID genetic  carriers/disorders  GENE THERAPY      ID mutant  genes;  purify replace m e nts  PHARMACEUTICAL PRODUCTION      Bacterial  production  of insulin,        Human  Growth  hormone  FORENSICS/PATERNITY     Crime  scene  analysis      Who’s  the  daddy?  GENETICALLY MODIFIED   ORGANISMS      “Golden”  rice- gene  for Vitamin  A added       Bt-corn  -resists  insect  pests        “frost resistant” strawberries       toxin/pollution  “eating”  bacteria  •  ENDANGERED  SPECIES  ZOO        cloning  extinct/endangered   species  CANARY  DETECTORS   PLASMIDS  = small  self replicating  DNA loops  Can  carry  genes  for ANTIBIOTIC  RESISTANCE  (used  as  genetic  markers •Used  as  VECTORS  to carry  recombinant  DNA  Can be cut with RESTRICTION ENZYMES Used to incorporate foreign DNA into bacteria Bacteria then reproduce, copying the inserted gene along with the plasmid Ways to make bacteria able to “take up” foreign DNA = “ COMPETENT” 1) ELECTROPORATION - zap with electricity
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