BISC 330 Spring 2011 Lecture 9

BISC 330 Spring 2011 Lecture 9 - BISC 330L: Biochemistry...

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ISC 330L: Biochemistry BISC 330L: Biochemistry ring Semester 2011 Spring Semester 2011 USC Lecture 9: Monday, 31 January 2011 1. Chapter 3: Biochemistry Toolbox chniques in Protein Analysis ¾ Techniques in Protein Analysis ¾ Determining Protein Sequence
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Why are amino acid sequences important? an make comparisons to other known ¾ Can make comparisons to other known proteins–Proteom ics eveal evolutionary pathways ¾ Reveal evolutionary pathways ¾ Search for internal repeats ¾ Can say something about destination or processing of protein ¾ Prepare antibodies – allow for further analysis ¾ Can make DNA probes
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Determining amino acid iti composition ¾ Peptide hydrolyzed in M HCl at 110 for 6M HCl at 110 o C for 24 h n exchange ¾ Ion exchange chromatography entity revealed by ¾ Identity revealed by elution volume uantity revealed by ¾ Quantity revealed by Ninhydrin (10 nmol) ¾ Can also use Fluorescamine (10 pmol)
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Protein Sequence Determination: dman Degradation Edman Degradation ¾ It is useful to know the specific amino acid sequence of a protein ¾ Edman Degradation: method developed in the 1950s by Pehr Edman to determine protein sequence ¾ With genome sequencing, this method is not as widely used.
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BISC 330 Spring 2011 Lecture 9 - BISC 330L: Biochemistry...

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