Perin First hour 1

Amnioc fluid cells amniotic fluid cell populations

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: ell types RT-PCR and Western Blogng: PRESENCE Real Time PCR: AMOUNT Range of analysis: 15-20 weeks of gesta&on (Da Sacco et al, 2010) Morphology of cells obtained from AF -ac&n osteopon&n sm ac&n cytokera&n neurofilam. ICAM AF Characteriza=on RT-PCR and Western Blogng confirmed the overall trend of expression of the different markers. Pluripotent markers OCT-4, C-kit and CD90 were expressed at any &me point CD34 was expressed only in samples older than 18 weeks Ectodermal markers expression was spreaded through all the considered weeks of gesta&on Mesodermal and Endodermal markers rarefied aKer 17 weeks of gesta&on 3. Stem Cells from Amnio&c Fluid (AFSC) Isola=on of stem cells from AF Samples are obtained via amniocentesis (14-20 week of gesta&on) Normal karyotype Normal cell cycle Can be cloned Expression of mesenchymal markers AFSC are seeded and cultured with specific differen&a&on media (serum , an=bio=c, growth factors, hormones) The modula=on and the different concentra=on of the growth factors permit the passage from an undifferen=ated state to a differen=ated one Skeletal muscle d. Osteogenic d. Endothelial d. Adipogenic d. Hepa=c d. Neuronal d. The most important demonstra&on of the pluripoten&ality of a stem cell is to inves&gate and prove if it par&cipates to the forma&on of &ssues during embr yo development Amnio=c fluid from C57BL/C6 Isola=on, expansion, Lac-Z and GFP infec=on ANALYSIS OF THE EMBRYO Injec=on of the blastocysts into a host female Blastocyst injec=on (3.5gg) Chimeric mouse (E 18): In situ detec&on of LacZ and GFP expression 4. Embryonic Kidney and Tissue Engineering Development biology seeks to study the gene&c control of cell growth , differen&a&on and morphogenesis, which is the process that gives rise to &ssues and organs Researchers are focusing more on stem cells in the hope of discovering beker applica&ons for bioengineering new &ssue in the labor...
View Full Document

This note was uploaded on 02/15/2012 for the course BME 410 taught by Professor Han during the Fall '08 term at USC.

Ask a homework question - tutors are online