Yu_Presentation-Optogenetic Control of Cardiac Function_11-17

Yu_Presentation-Optogenetic Control of Cardiac Function_11-17

Info iconThis preview shows pages 1–14. Sign up to view the full content.

View Full Document Right Arrow Icon
Optogenetic Control of Cardiac Function Aristides B. Arrenberg, et al. Science 330, 971 (2010);
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Introduction The cardiac pacemaker can be substituted by a battery-operated device. This group created pacemaker by expressing halorhodopsin and channelrhodopsin in zebrafish cardiomyocytes. They combined optogenetics and light-sheet microscopy to reveal the emergence of organ function during development.
Background image of page 2
Zebrafish expressing NpHR The light gated pump NpHR-mCherry was expressed in zebrafish cardiomyocytes by means of theGal4/UAS system.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Automated mapping of the cardiac pacemaker using patterned illumination
Background image of page 4
Automated mapping of the cardiac pacemaker using patterned illumination
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Location of the pacemaker cells in sinoatrial ring (SAR) and atrioventricular canal (AVC) cells
Background image of page 6
Automated mapping of a 3 dpf heart Ventricle Atrium Illumination induced local hyperpolarization and ceased contraction Heart stopped when SAR was illuminated (Position 6) and AV block was
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 8
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 10
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 12
Background image of page 13

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 14
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: induced when AVC was illuminated (Position 8) Illumination of SAR cells (as few as only 3) can block heartbeat Using this Method to Induce Cardiac States Reversed cardiac conduction induced by ChR2 This method can be used to quickly manipulate the zebrafish heart and switch between healthy and diseased states Illuminating the AVC (atrioventricular canal) with different light intensities generated AV blocks with different severities Setup Results AV Ratio = Ratio of non-conducted to conducted beats Heart Rate Pacing Illuminating the SAR (sinoatrial ring) with short pulses of light Vary the frequency of the pulses to control the heart rate Results Conclusion and Implications This is a very powerful tool for in vivo control of disease-like states There are a few select pacemaker cells which are indispensable for embryonic heartbeat initiation We can use this to control hemodynamic states while looking at blood vessel development...
View Full Document

Page1 / 14

Yu_Presentation-Optogenetic Control of Cardiac Function_11-17

This preview shows document pages 1 - 14. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online