Lecture 26d BioMEMS

Lecture 26d BioMEMS - PDMS molds Cell Encapsulants •...

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Tissue Engineering BioMEMS Shortcourse Dr. Bruce K. Gale Cell Culture Results • Cells Cultured on PDMS Substrates (Plus-sign shaped structure) – Cell density in the smooth areas was only slightly higher than that in the microstructures – Two ends of cells attach to the two sidewalls of the corner – Cells migrate to the locations of highest surface area and then expand from there Peg Scaffold • PDMS cell culture scaffold • Pegs with height 5 microns, diameter 10 microns • Pegs spaced 20 microns center-to-center, in rows, 30 microns between rows • Surface coated with laminin • Cells grown in standard cell cultures are: – Not oriented properly – Weakly adherent to the substrate – Not 3-dimensional Peg Results • Cells frequently terminated with a blunt end on a peg • Tendency for cells to straddle between rows Cardiac myocytes cultured on silicone structure
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Scaffolds Using Stacked 2-D Structures Stacks of microstructures for tissue engineering Fabricated using polymer injection method
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Unformatted text preview: PDMS molds Cell Encapsulants • Encapsulated cells protected from immune system – Antibodies repelled • 18 nm pores fabricated using sacrificial oxide • Encapsulated islet cells survive and respond to glucose levels Functional Capillaries on a Chip • 10 micrometer lines induce capillary formation in endothelial cells Controlled Cell Death • Cell areas linked to apoptosis (cell suicide) Chain Mail for Bone Engineering Multi layer polysilicon process Made using metals, polysilicon, and SiO 2 MG-63 cells 24 post-seeding 80° orientation MG-63 cells 24 post-seeding 80° orientation 500 µ m diameter 10 µ m ring width Local flexibility and general rigidity are the primary advantages Uniform seeding also possible MG-63 cells 24 post-seeding 80° orientation MG-63 cells 24 post-seeding 80° orientation 500 µ m diameter 10 µ m ring width µ m ring width Cellular processes Cellular processes...
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This note was uploaded on 02/14/2012 for the course BIOE 6421 taught by Professor Gale during the Fall '11 term at UVA.

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Lecture 26d BioMEMS - PDMS molds Cell Encapsulants •...

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