{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Lecture 19-LC710 - Maxim-Gilbert Sequencing YNPV Logo for...

Info iconThis preview shows pages 1–36. Sign up to view the full content.

View Full Document Right Arrow Icon
Plasmid (BAC) Phagemid Cosmid Lambda (PAC) Lots of tools for cloning:
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Background image of page 2
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Needs to be at least 40kb to grow, but A left and right arm can be made smaller… See next slide
Background image of page 4
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Background image of page 6
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Intron and Exon in Eukaryotic Cells mRNA DNA 5’ 3’ cap poly A tail exon exon exon intron intron mature mRNA Processing Transcription Splicing promotor 5’ 3’ Take place in nucleus start codon stop codon To cytoplasm Intron deleted
Background image of page 8
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Eukaryotes Splice Signals AG/GT AG/GT CAG/NT CAG/NT exon 1 intron 1 exon 2 exon 1 intron 1 exon 2
Background image of page 10
cDNA Is Reverse Transcribed from mRNA mature mRNA poly A tail 5’ 3’ TTTT Reverse transcription CCC 3’ 5’ 3’ 5’ 3’ GGG DNA polymerase RNA hydrolysis 5’ 3’ 5’ Baltimore, Dulbecco, Temin (1975)
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
cDNA Library mRNA cDNA Reverse transcription Genes in expression Complete gene Vector: Plasmid
Background image of page 12
Libraries: cDNA cDNA library has sequences only from genes transcribed into mRNA (~3% of genome)
Background image of page 13

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Background image of page 14
Background image of page 15

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Background image of page 16
MNGTEGPNFYVPFSNKTGVVRSPFEAPQYYLAEPWQFSMLAAYMFLLIML
Background image of page 17

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Background image of page 18
Background image of page 19

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Background image of page 20
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: GFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLH GYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGE NHAIMGVAFTWVMALACAAPPLVGWSRYIPEGMQCSCGIDYYTPHEETNN ESFVIYMFVVHFIIPLIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEV TRMVIIMVIAFLICWLPYAGVAFYIFTHQGSDFGPIFMTIPAFFAKTSAV YNPVIYIMMNKQFRNCMVTTLCCGKNPLGDDEASTTVSKTETSQVAPA* Maxim-Gilbert Sequencing MNGTEGPNFYVPFSNKTGVVRSPFEAPQYYLAEPWQFSMLAAYMFLLIML GFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLH GYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGE NHAIMGVAFTWVMALACAAPPLVGWSRYIPEGMQCSCGIDYYTPHEETNN ESFVIYMFVVHFIIPLIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEV TRMVIIMVIAFLICWLPYAGVAFYIFTHQGSDFGPIFMTIPAFFAKTSAV YNPV IYIMMNKQFRNCMVTTLCCGKNPLGDDEASTTVSKTETSQVAPA* Logo for a) Splice acceptor b) Splice Donor c) Initiator Met AG/GT AG/GT CAG/NT CAG/NT exon 1 intron 1 exon 2 exon 1 intron 1 exon 2...
View Full Document

Report

{[ snackBarMessage ]}