Lecture 21 -LC710 1st hr

Lecture 21 -LC710 1st hr -...

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Plasmid (BAC) Phagemid Cosmid Lambda (PAC) Lots of tools for cloning:
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Needs to be at least 40kb to grow, but A left and right arm can be made smaller… See next slide
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MNGTEGPNFYVPFSNKTGVVRSPFEAPQYYLAEPWQFSMLAAYMFLLIML GFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLH GYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGE NHAIMGVAFTWVMALACAAPPLVGWSRYIPEGMQCSCGIDYYTPHEETNN
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Unformatted text preview: ESFVIYMFVVHFIIPLIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEV TRMVIIMVIAFLICWLPYAGVAFYIFTHQGSDFGPIFMTIPAFFAKTSAV YNPVIYIMMNKQFRNCMVTTLCCGKNPLGDDEASTTVSKTETSQVAPA* Logo for a) Splice acceptor b) Splice Donor c) Initiator Met AG/GT AG/GT CAG/NT CAG/NT exon 1 intron 1 exon 2 exon 1 intron 1 exon 2...
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This note was uploaded on 02/14/2012 for the course BIO 710L taught by Professor Goldfarb during the Fall '10 term at Iowa State.

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Lecture 21 -LC710 1st hr -...

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