Quiz 3 - What will you change Arg53 to 5’ 3’ Question...

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Question 1: Name:_______________ Quiz 3: Biol 302 Spring2011 Draw and Label a Two Exon Complete Gene. 10pts -labeling 14 structures correctly, 8pts -labeling 10-13 structures correctly, 5pts -labeling 6-9 structures correctly. 0pts for less than 6 structures labeled correctly or for any inaccuracies. Question 2: The two differences between splicing of a transcript and gene recombination are: 1pt _______________________________ 1pt _______________________________ 20pts total Given the following DNA binding motif: Helix2-turn-Helix3 for a homeodomain protein. Question 3: Trp48, Phe49, Asn 51 and Arg53 are invariant amongst almost all homeodomains in order to retain DNA binding. However, you want to test this idea with single amino acid substitutions. (2pts for 3 correct) What will you change Trp48 to: _____ What will you change Phe49 to: _____ What will you change Asn51 to: _____
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Unformatted text preview: What will you change Arg53 to: _____ 5’ 3’ Question 4: : Given the following patterns produced in Drosophila segments starting from #1(WT) GS A1 A2 A2 A2 A2 A2 A2-AbdA Ubx AbdA AbdA AbdA AbdA AbdA #1 Minus Antp #1 plus overexpression of Antp in all segments, 2pts #1 plus overexpression of Ubx in all segments, 2pt s #1 plus overexpression of AbdA in all segments, 2pts Fill in appropriate segment identity in boxes below. Extra Credit 2pts : The above results of segment identity can be explained in two ways T2 T2 A2 A2 A2 A2 A2 A2 Antp AbdA Antp AbdA AbdA AbdA AbdA AbdA #1 minusUbx T2 A1 A2 A2 A2 A2 A2 A2 Antp AbdA Ubx AbdA AbdA AbdA AbdA AbdA #1 Either the _____________________________________________________ or ________________________________________________________________________ WT...
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This note was uploaded on 02/14/2012 for the course BIO 710L taught by Professor Goldfarb during the Fall '10 term at Iowa State.

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Quiz 3 - What will you change Arg53 to 5’ 3’ Question...

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