Quiz3 Fall 2011

Quiz3 Fall 2011 - LC710 11/09/11 name____________ You make...

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You plan to identify this gene in mouse using ( 2pts ) _______________________as a PCR template. Design 5’ to 3’ oligos that will give you 50% or better chance at identifying this coding sequence in the mouse. You are fearful of getting to much garbage from your amplification MNGTYERATLSMLWAAYMFLLIML * Q1(8 pts) You have just purified this protein from the eyes of the fish Fugu: * = STOP (4pt) How will you know that Your PCR succeeded in amplifying the correct fragment (be specific)? Quiz#3
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Unformatted text preview: LC710 11/09/11 name____________ You make an antibody to this protein and find that it is only found in the eyes of fish. You look for that protein in mouse tissues and again it is only found in the eye. 1 2 1 2 1 2 1 2 Codon Probability In fish 1 In mouse 2 organisms _____________________________________ _____________________________________ _____________________________________ (1pt) Oligo2:______________________________ Oligo1:_______________________________ (1pt)...
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