Quiz6 Fall2011

Quiz6 Fall2011 - Using standard PCR amplification: What...

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Quiz#6 LC710 11/21/11 name___________ Q3: Given the following eukaryotic gene A : A B C D Intron size: 1 Million bp Intron size: 2 Million bp Intron size: 100 bp 1 2 3 4 5 7 6 8 Using standard PCR amplification on DNA from: gDNA cDNA Primers: 1-2 _____ _____ ______ 1-3 _____ _____ ______ 1-4 _____ _____ ______ 3-4 _____ _____ ______ 3-6 _____ _____ ______ 5-7 _____ _____ ______ 5-8 _____ _____ ______ Boxes A, B, C, and D are all 100bp. All primers begin at the ends of those boxes.
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Unformatted text preview: Using standard PCR amplification: What size band would you find on your gel for each primer pair? 1pt for each correct row NOT DRAWN TO SCALE mix of gDNA and cDNA Q1: Can you PCR amplify the Entire E.Coli genome with 2 primers? ( 1pt ) Circle one: yes/no to Q1 (1pt) : Quiz 5 distribution: I see 8 obvious A students! Overall distribution: I see 9 obvious A students!...
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This note was uploaded on 02/15/2012 for the course BIO 710L taught by Professor Goldfarb during the Fall '10 term at Iowa State.

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Quiz6 Fall2011 - Using standard PCR amplification: What...

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