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Unformatted text preview: 5-CATTCAAATATGTATCCGAGCTAGCTTAATTGAGCTAGCTGATAAATGCTTCAATAAT-3 5-ATTATTGAAGCATTTATCAGCTAGCTCAATTAAGCTAGCTCGGATACATATTTGAATG-3 (1pt) What is the 6 nucleotide consensus sequence: _ _ _ _ _ _ (1pt) What did you have to do in order to find the consensus? _______________________ (be specific) (1pt) What is odd about your result?:____________________________________________ Q2: You set up the following one-hybrid screening system using the consensus sequence : Draw an arrow as to where you would place your consensus sequence In the plasmid below. Is there a benefit to putting more than 1 site (1pt)? _____________________________________________________________ Plasmid above has a minimal promoter (TATA box) What does the red box represent in this experiment (1pt)?___________________________ Q3: Using the 1000 different cDNAs from Q1, please describe what you will fuse them to and why?(2pts) (be specific)...
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