Quiz8 Fall2011

Quiz8 Fall2011 - A thus causing Protein A to bind to...

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Quiz#8 LC710 11/28/11 name___________ Q1: Given the following activity pattern for your enhancer/promoter complex: β- galactosidase activity A B C D LacZ E F 1000 A B C D LacZ E 900 A B LacZ 0 C D LacZ 0 LacZ E F 0 LacZ 200 B C D A E LacZ 100 Make a conclusion about the enhancers as to how they work. Be Brief 4pts (1 sentence) Points will be taken off for being too descriptive. _______________________________________________________________________ _______________________________________________________________________
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A B X Q2: Given the following scenario: Three Eukaryotic proteins where Estrogen binds to Protein
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Unformatted text preview: A, thus causing Protein A to bind to Protein X, thus causing Protein X to bind to Protein B. Protein B is a DNA binding protein and typically found in the nucleus. Protein A and X can be found in the cytosol and nucleus. The DNA element Z has been identified as the binding site for Protein B. Describe (and draw) an in vivo assay that would confirm this scenario ( 4pts ) E.C. ( 1pt ) Why might you want to add Estrogen in your assay? _________________________________________________________________________...
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Quiz8 Fall2011 - A thus causing Protein A to bind to...

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