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Quiz9 Fall2011 - 2-3pts You hypothesize that splicing is...

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Quiz#9 LC710 11/30/11 name___________ A B C D Intron size: 1 Million bp Intron size: 2 Million bp Intron size: 100 bp 1 2 3 4 5 7 6 8 Boxes A, B, C, and D are all 100bp. All primers begin at the ends of those boxes (each primer is 20nts long). NOT DRAWN TO SCALE Q1: Given the following Eukaryotic gene: You have in your possession oligos 1 to 8 and gDNA. You have a patient with a disease in one of these exons. Approximately what percentage (fraction) of the coding sequence can be determined using these oligos for PCR and sequencing?________________________ (
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Unformatted text preview: 2-3pts ). You hypothesize that splicing is part of the problem. Using these oligos, what will you be able to determine?_________________________________________________( 2pts ) Briefly describe how you would determine the maximum number of mutations in C and D ( 4pts )? Be specific: _______________________________________________________________ _______________________________________________________________________ _______________________________________________________________________...
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