Quiz11 - 1 gene. You do not have an antibody available to...

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Quiz#11 LC710 11/01/10 name___________ You have just identified a point mutation in mice; Rho 1 If the mice are Rho 1 /Rho 1 , then they are blind. You think that the reason these mice are blind is because they fail to express Rhodopsin. (2pts) Briefly design a non-inducible transgenic mouse experiment. Q2 (2pts) Briefly design an inducible (post birth) transgenic mouse experiment. Q3 (2pts) Briefly design an inducible (post birth) transgenic mouse experiment that you can vary the amount of protein produced based on the amount of Doxycycline you feed to the animal. Showing constructs and some explanation, evaluate if Rhodopsin encodes the Rho
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Unformatted text preview: 1 gene. You do not have an antibody available to the Rhodopsin protein. Thus, make sure you are able to assess that the protein is being made . You want to characterize the effects on ablating a population of cells in the retina . Fortunately you have the Rhodopsin promoter and the following constructs to put in the mouse. Rhodopsin p Q4 (4pts) ROSA (ubiquitous promoter) Please put DNAs of your choice (describe what they are) where Red arrows point. Please label 4 boxes with ORF names and direction of those ORFs Please describe how the experiment will run. What will you see?...
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This note was uploaded on 02/15/2012 for the course BIO 710L taught by Professor Goldfarb during the Fall '10 term at Iowa State.

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Quiz11 - 1 gene. You do not have an antibody available to...

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