Exam 2009 - LS4 Final Exam-Fall 2009 Name: University ID:...

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LS4 Final Exam—Fall 2009 Name: University ID: Instructions: There are 10 problems on this exam. Each is worth 15 points. 1. Make your answers clear and concise. 2. Use the back of each sheet to work out the problems 3. Make it clear what your final answer is. Circling them helps. 4. Write in blue or black ink only. 5. Do not write in the boxes. They are for grading purposes. Score: Problem 1 Problem 6 Problem 2 Problem 7 Problem 3 Problem 8 Problem 4 Problem 9 Problem 5 Problem 10 Total
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Name: UID: Problem 1 While working in the lab, you find six deletion mutants of Neurospora crassa that are auxotrophic for lysine. The A and α spores for each mutant are mated with each other to form a dikaryon (a diploid cell) and plated on minimal media. A) Assuming you made no errors while performing the crosses, fill in the expected outcomes of yet to be completed complementation tests. (+ = growth on minimal media, - = no growth on minimal media) ( 4 points ) 1 2 3 4 5 6 1 - - + 2 - + - 3 - - 4 - + 5 - + 6 - How many genes are important for lysine production? ( 1 point ) ____________ B) You find four new mutants in the same complementation group that are auxotrophic for lysine and appear to be deletions, and cross these mutants pair wise to make diploids that are heterozygous for both deletions. You induce recombination in these mitotically dividing diploid cells by exposing them to UV light, and find that now some cells can grow on minimal media. Construct a map of the four deletions based on results in the table below. (+ = growth on minimal media, - = no growth on minimal media) ( 4 points ) I II III IV I - + - + II + - - - III - - - + IV + - + - Problem continues on the next page
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Name: UID: C) You were also able to find 6 point mutants (a-f) in the same gene that were also lysine auxotrophs. You crossed them with the deletions found in part B and plated the diploids on minimal media. The results are shown in the chart below. Construct a map with both deletions and point mutations. ( 4 points ) a b c d e f I + + - + + + II - - + + - + III - + - - + - IV + - + + + + D) The relative locations of point mutants d and f cannot be distinguished from the experiments you’ve done. How could you do an experiment, involving recombination, to distinguish the order of d and f on the chromosome? Describe this briefly. ( 2 points ) (Hint: you’ll have to use two different diploids in this experiment)
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Name: UID: Problem 2 Malaria infects humans by first infecting mosquitoes, which then pass on the disease to humans. Some mosquitoes have a recessive allele of a gene that when homozygous confers resistance to malaria. Mosquitoes with this genotype do not pass malaria on to humans. In a certain population, 10,000 out of 100,000 mosquitoes were unable to transmit malaria. A) Assuming Hardy-Weinberg equilibrium, what is the frequency of the resistance allele?
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This note was uploaded on 02/15/2012 for the course PMB 13 taught by Professor Freeling during the Spring '09 term at University of California, Berkeley.

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Exam 2009 - LS4 Final Exam-Fall 2009 Name: University ID:...

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