PS 1 answers, prob 13

PS 1 answers, prob 13 - your mutants from above on minimal...

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Problem 13. You discover a new compound that yeast require for survival. To find genes involved in the synthesis of this compound (compound X) you have screened for mutants that cannot grow on minimal media, but can grown on minimal media + compound X. You find 10 mutants and perform a complementation tests. The results are shown below (+ = growth, - = no growth). 1 2 3 4 5 6 7 8 9 10 1 - + + - + + + + + + 2 - - + + + - + + + 3 - + + + - + + + 4 - + + + + + + 5 - - + + - + 6 - + + - + 7 - + + + 8 - + - 9 - + 10 - A) How many genes are in your mutant collection? 4 genes B) Which mutations are in the same genes? 1, 4 2, 3, 7 5, 6, 9 8, 10 You know that the earliest precursor for compound X is compound Y. There are 5 other compounds that could potentially be involved in the pathway (L, M, N, O, P). To determine if, and in what order, these compounds are involved in the pathway you grow
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Unformatted text preview: your mutants from above on minimal media + one of the compounds. The results are below. L M N O P X Y 1 - - + - - + - 2 - + + - - + - 3 - + + - - + - 4 - - + - - + - 5 - + + - + + - 6 - + + - + + - 7 - + + - - + - 8 - - - - - + - 9 - + + - + + - 10 - - - - - + - Problem continues on the next page C) Given the results reported in the chart on the previous page, draw the biosynthetic pathway for compound X indicating which mutations correspond to which genes in the pathway. 5,6,9 2,3,7 1,4 8,10 Y ! P ! M ! N ! X D) You have constructed several double mutant strains. Indicate whether or not these double mutants would grow on minimal media + the following compounds. ( 3 points ) L M N O P X Y 1, 2 - - + - - + - 1, 4 - - + - - + - 5, 10 - - - - - + -...
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This note was uploaded on 02/15/2012 for the course PMB 13 taught by Professor Freeling during the Spring '09 term at Berkeley.

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PS 1 answers, prob 13 - your mutants from above on minimal...

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