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PS 1 answers, prob 12

PS 1 answers, prob 12 - Problem 12 While working in the lab...

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Problem 12. While working in the lab, you find six deletion mutants of Neurospora crassa that are auxotrophic for lysine. The A and ! spores for each mutant are mated with each other to form a dikaryon (a diploid cell) and plated on minimal media. A) Assuming you made no errors while performing the crosses, fill in the expected outcomes of yet to be completed complementation tests. (+ = growth on minimal media, - = no growth on minimal media) ' ( ) * + , ' - - . . . - ( - . . . - ) - . - . * - . . + - . , - How many genes are important for lysine production? _____ 3 _______ B) You find four new mutants in the same complementation group that are auxotrophic for lysine and appear to be deletions, and cross these mutants pair wise to make diploids that are heterozygous for both deletions. You induce recombination in these mitotically dividing diploid cells by exposing them to UV light, and find that now some cells can grow on minimal media. Construct a map of the four deletions based on results in the table below. (+ = growth on minimal media, - = no growth on minimal media) ( 4 points ) / // /// /0 / - . - .
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