PS 1 answers, probs 3+4 - the same gene. Mutations 2 and 4...

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Problem 3. You have performed a mutagenesis screen in Drosophila and have found 5 recessive loss-of-function mutants with an essential role in the neural pathway that allows flies to jump. You perform a complementation test and get the following results. + = wildtype, - = non-jumpers There are three complementation groups Group 1: mutations 1, 3, and 4 Group 2: mutation 2 Group 3: mutations 5 and 6 Problem 4. Four rII- recessive point mutations were discovered. Coinfections of bacteria with all possible combinations of rII- mutant T4 phages were performed, and the following results were obtained. Fill in the chart 1 2 3 4 1 - + - + 2 - + + 3 - + 4 - A. How many genes are identified by this analysis? From row 1, we see that mutation 1 fails to complement mutation 3, so they are in
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Unformatted text preview: the same gene. Mutations 2 and 4 complement mutation 1, so they are not in the same gene as mutation 1. 2 and 4 are not necessarily in the same gene, we must keep looking at the chart to figure that out. All we know so far is that mutations 2 and 4 are not in the same gene as mutation 1 and 3. From row 2, we see that mutation 2 complements mutation 4, so they must also be in different genes. B. Which mutants belong to the same complementation groups? 1 complementation group (mutations in same gene) mutations 1 and 3 mutation 2 is in a different gene than 4 and (1 and 3) mutation 4 is in a different gene than 2 and (1 and 3)...
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This note was uploaded on 02/15/2012 for the course PMB 13 taught by Professor Freeling during the Spring '09 term at University of California, Berkeley.

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