ps1 answers, probs 1+2

ps1 answers, probs 1+2 - Most eukaryotes are diploid or are...

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LS4 Problem Set 1, 2010 Complementation testing, fine mapping by recombination, biochemical pathways, reading frames, mutations Corresponding Lectures: October 30, November 2, 4, 6 Corresponding Reading: Hartwell et al., 224-238, 255-265 Corresponding Quizzes: Nov 2-6 (simple quiz on complementation); Nov 9-13 If you want additional problems, try these from the book: Chapter 7: 16-19, 21, 23, 32; Chapter 8: 3-7, 16. Problem 1. 1. What is the purpose of a complementation test? To determine if two mutations with the same phenotype occur in the same gene or in different genes 2. What is one criterion that must be met in order for a complementation test to be a valid experiment? Mutations must be recessive 3. How do complementation tests differ between animals, bacteria and viruses?
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Unformatted text preview: Most eukaryotes are diploid or are sometimes diploid as a part of their life cycle (Also accept easier to score phenotypes) 4. Is this an example of complementation or of failure to complement? Complementation Problem 2. You have 3 turtles, each with a mutation that causes them to have a strange polka dotted pattern on their shells. You don’t know if the mutations are in the same gene or in different genes. Assume that all 3 mutations are homozygous recessive. a) turtle1 X turtle2 turtle2 X turtle3 turtle3 X turtle1 b) Turtle3 x turtle2 Complementation, wild type phenotype Turtle1 x turtle3 No complementation, mutant phenotype...
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This note was uploaded on 02/15/2012 for the course PMB 13 taught by Professor Freeling during the Spring '09 term at Berkeley.

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