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ps3, prob 10 - sequence for the Super Sweaty allele...

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Problem 10. Super Sweaty syndrome is a genetic disorder that results from a missense mutation. The only difference between the disease and standard alleles is a substitution of A for C in the in the middle position of a codon, which codes for alanine in the standard protein and aspartic acid in the mutant allele. The mutation abolishes a site for the restriction enzyme DryII (DryII recognizes the sequence CGATCG). Standard copies of the allele can be cut with this enzyme resulting in 100, 300 and 700 base pair fragments. Mutant alleles lack one site resulting in 100 and 1000 base pair fragments. A) Given the sequence fragment from the standard allele below, write the corresponding
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Unformatted text preview: sequence for the Super Sweaty allele. Standard allele: 5’-ATGGCACCCGATCGATTCGCTACTGA-3’ Super Sweaty allele: B) Below is a pedigree of a family that is afflicted with Super Sweaty syndrome. Is this a recessive, dominant, or X-linked disease? I II III IV 1 2 3 4 5 6 7 8 9 10 11 12 13 14 C) Fill in the bands on the gel below for as many family members as possible. MW 1 2 3 4 5 6 7 8 9 10 11 12 13 14 D) For which individual(s) in the pedigree can the genotype not be known? What could each of their genotypes be? 500bp 700bp 1000bp...
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