ps3, probs 5+6

ps3, probs 5+6 - Problem 5. K has found a mutant fly that...

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Problem 5. K has found a mutant fly that flies upside-down. She decides to try to clone the mutant gene positionally. She finds 4 microsatellite loci that differ between the mutant and a wild-type strain, and calls them A, B, C, and D. She crosses the mutant to a wild-type fly. The genotype of the mutant fly at the 4 microsatellites is: AA, BB, CC, DD; the genotype of the wild-type fly is aa, bb, cc, dd. All of the F1 progeny from this cross fly normally. She crosses the F1 to each other, and finds that ! of the next generation flies upside-down. She collects DNA from 12 of these mutants and performs PCR to genotype all of the microsatellites in these 12 animals. The results of her genotyping are below. Mutant flies 1 2 3 4 5 6 7 8 9 10 11 12 Genotypes Aa Aa aa Aa AA AA aa Aa aa Aa Aa AA BB BB Bb Bb BB BB BB BB BB BB BB BB Cc cc Cc Cc CC CC Cc cc Cc Cc Cc cc Dd dd Dd Dd DD DD Dd dd Dd Dd dd Dd a)
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This note was uploaded on 02/15/2012 for the course PMB 13 taught by Professor Freeling during the Spring '09 term at Berkeley.

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