ps3, probs 3+4

ps3, probs 3+4 - A and C can be cut in the mutant allele,...

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Problem 3. In mouse Hind III restriction digests, a RFLP has two alternative alleles: one that creates 2.7 and 1.1 kb bands, and one that doesn’t cut and therefore gives one 3.8 kb band. A mouse heterozygous for a dominant allele for bent tail and the above RFLP is mated to a wildtype mouse that has only the 3.8 kb-fragment. Thirty percent of the bent-tail progeny are homozygous for the 3.8 kb allele, and 70 percent have both alleles (and therefore three bands). a. Is the bent-tail locus linked to the RFLP locus? Explain your answer. b. What RFLP types do you predict among the wild-type offspring, and in what proportions? Problem 4 . A 6Kb PCR amplified fragment includes three RFLPs in a family that is affected by a disease. The restriction sites in the RFLPs are labeled A, B, and C.
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Unformatted text preview: A and C can be cut in the mutant allele, but not the normal allele. B can be cut in the normal allele, but not the mutant allele. The restriction cut sites are mapped on the PCR fragment as follows: ( 6 Kb total ) You do a restriction digest on the PCR products of each of your family members. Based on the gel below and the map above, what are the genotypes of each family member homozygous mutant (-/-), heterozygous carrier (+/-), or homozygous normal (+/+)? mwm mom dad child 1 child 2 child 3 child 4 child 5 6 Kb 5 Kb 4 Kb 3 Kb 2 Kb 1 Kb _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ genotype A B C 2 Kb 2Kb 1 Kb 1 Kb...
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This note was uploaded on 02/15/2012 for the course PMB 13 taught by Professor Freeling during the Spring '09 term at Berkeley.

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