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Unformatted text preview: determine if, and in what order, these compounds are involved in the pathway you grow your mutants from above on minimal media + one of the compounds. The results are below. L M N O P X Y 1 - - + - - + - 2 - + + - - + - 3 - + + - - + - 4 - - + - - + - 5 - + + - + + - 6 - + + - + + - 7 - + + - - + - 8 - - - - - + - 9 - + + - + + - 10 - - - - - + - Problem continues on the next page C) Given the results reported in the chart on the previous page, draw the biosynthetic pathway for compound X indicating which mutations correspond to which genes in the pathway. D) You have constructed several double mutant strains. Indicate whether or not these double mutants would grow on minimal media + the following compounds. ( 3 points ) L M N O P X Y 1, 2 1, 4 5, 10...
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This note was uploaded on 02/15/2012 for the course PMB 13 taught by Professor Freeling during the Spring '09 term at University of California, Berkeley.
- Spring '09