ps1, prob 12 - results in the table below. (+ = growth on...

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Problem 12. While working in the lab, you find six deletion mutants of Neurospora crassa that are auxotrophic for lysine. The A and ! spores for each mutant are mated with each other to form a dikaryon (a diploid cell) and plated on minimal media. A) Assuming you made no errors while performing the crosses, fill in the expected outcomes of yet to be completed complementation tests. (+ = growth on minimal media, - = no growth on minimal media) " # $ % ' " ( ( ) # ( ) ( $ ( ( % ( ) ( ) ' ( How many genes are important for lysine production? ____________ B) You find four new mutants in the same complementation group that are auxotrophic for lysine and appear to be deletions, and cross these mutants pair wise to make diploids that are heterozygous for both deletions. You induce recombination in these mitotically dividing diploid cells by exposing them to UV light, and find that now some cells can grow on minimal media. Construct a map of the four deletions based on
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Unformatted text preview: results in the table below. (+ = growth on minimal media, - = no growth on minimal media) ( 4 points ) * ** *** *+ * ( ) ( ) ** ) ( ( ( *** ( ( ( ) *+ ) ( ) ( Problem continues on the next page C) You were also able to find 6 point mutants (a-f) in the same gene that were also lysine auxotrophs. You crossed them with the deletions found in part B and plated the diploids on minimal media. The results are shown in the chart below. Construct a map with both deletions and point mutations. ,-. / 1 * ) ) ( ) ) ) ** ( ( ) ) ( ) *** ( ) ( ( ) ( *+ ) ( ) ) ) ) D) The relative locations of point mutants d and f cannot be distinguished from the experiments youve done. How could you do an experiment, involving recombination, to distinguish the order of d and f on the chromosome? Describe this briefly. (Hint: youll have to use two different diploids in this experiment)...
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This note was uploaded on 02/15/2012 for the course PMB 13 taught by Professor Freeling during the Spring '09 term at University of California, Berkeley.

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ps1, prob 12 - results in the table below. (+ = growth on...

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