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Unformatted text preview: results in the table below. (+ = growth on minimal media, - = no growth on minimal media) ( 4 points ) * ** *** *+ * ( ) ( ) ** ) ( ( ( *** ( ( ( ) *+ ) ( ) ( Problem continues on the next page C) You were also able to find 6 point mutants (a-f) in the same gene that were also lysine auxotrophs. You crossed them with the deletions found in part B and plated the diploids on minimal media. The results are shown in the chart below. Construct a map with both deletions and point mutations. ,-. / 1 * ) ) ( ) ) ) ** ( ( ) ) ( ) *** ( ) ( ( ) ( *+ ) ( ) ) ) ) D) The relative locations of point mutants d and f cannot be distinguished from the experiments youve done. How could you do an experiment, involving recombination, to distinguish the order of d and f on the chromosome? Describe this briefly. (Hint: youll have to use two different diploids in this experiment)...
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This note was uploaded on 02/15/2012 for the course PMB 13 taught by Professor Freeling during the Spring '09 term at University of California, Berkeley.
- Spring '09