ps1, prob 9 - B) You have mixed up the vials of your double...

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Problem 9 . The genes Arg-F, Arg-G, and Arg-H are required for arginine biosynthesis in neurospora, as indicated below. ARG-F ARG-G ARG-H Ornithine Citrulline Arginino-Succinate Arginine A) In the chart below, some single, double, and triple mutant haploid strains are indicated on the left. Indicate whether or not they will grow on minimal media supplemented with the compounds at the top of the chart. Put a check in each box when the strains can grow and leave the box blank when the strains can not grow. Compounds supplementing minimal media Mutant Strains Ornithine Citrulline Arg-Succ Arginine arg-F arg-G arg-H arg-F; arg-G arg-F, arg-H arg-G, arg-H arg-F, arg-G, arg-H
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Unformatted text preview: B) You have mixed up the vials of your double and triple mutant strains (which have been growing in complete media) and discover that there is one vial that you forgot to label. You find that spores from that vial grow on minimal media supplemented with arginine, but not on media supplemented with ornithine, citrulline or arginino-succinate. List all of the possible strains that this could be. C) You cross this mystery strain to the Arg-F single mutant to create a diploid. This diploid grows on minimal media. What was the mystery strain? D) If you cross the mystery strain to the Arg-G mutant, which compounds will the resulting diploid be able to grow on?...
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This note was uploaded on 02/15/2012 for the course PMB 13 taught by Professor Freeling during the Spring '09 term at University of California, Berkeley.

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