ps1, probs 7+8

ps1, probs 7+8 - What kind of test would you perform to...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Problem 7. You have four known deletion mutants for a single gene: W, X, Y and Z. Point mutations 1, 2, 3, 4, 5, and 6 are all mutations in the same complementation group. Based on the recombination tests shown in the table below, draw map showing the deletions and point mutations. Please make the map as accurate as possible. W X Y Z 1 + + - - 2 + + - + 3 - + + + 4 - + + - 5 + + - - 6 + - - + Problem 8. A) You are interested in the biochemical pathway for lysine production. You have gathered a collection of E. coli mutants by mutagenizing wild type bacteria and growing them in complete media. You then screened for auxotrophs by inoculating cultures in minimal media. How will you collect mutants that are auxotrophic for lysine? B) After isolating your lysine auxotrophs you wish to determine how many genes there are in the lysine biosynthetic pathway.
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: What kind of test would you perform to answer this question? C) Your test has determined that there are 4 genes in the lysine pathway, which is not surprising to you as you have biochemical data indicating the presence of what appears to be 4 precursors of lysine. You call the precursors A, B, C and D and your complementation groups 1, 2, 3 and 4. By growing each of your groups in minimal media supplemented with the indicated precursor you hope to order the precursors of the pathway and determine which step each of the mutants are deficient in. The following chart summarizes your data. Please indicate the order of the pathway and show which biochemical reaction each gene performs. Precursor Complementation group A B C D Lysine 1 + - + + + 2 - - + - + 3 + - + - + 4 - - - - +...
View Full Document

This note was uploaded on 02/15/2012 for the course PMB 13 taught by Professor Freeling during the Spring '09 term at Berkeley.

Ask a homework question - tutors are online