PS1, probs 1+2

PS1, probs 1+2 - (4) Is this an example of complementation...

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LS4 Problem Set 1, 2010 Complementation testing, fine mapping by recombination, biochemical pathways, reading frames, mutations Corresponding Lectures: October 30, November 2, 4, 6 Corresponding Reading: Hartwell et al., 224-238, 255-265 Corresponding Quizzes: Nov 2-6 (simple quiz on complementation); Nov 9-13 If you want additional problems, try these from the book: Chapter 7: 16-19, 21, 23, 32; Chapter 8: 3-7, 16. Problem 1. (1) What is the purpose of a complementation test? (2) What is one criterion that must be met in order for a complementation test to be a valid experiment? (3) How do complementation tests differ between animals, bacteria and viruses?
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Unformatted text preview: (4) Is this an example of complementation or of failure to complement? Problem 2. You have 3 turtles, each with a mutation that causes them to have a strange polka dotted pattern on their shells. You dont know if the mutations are in the same gene or in different genes. Assume that all 3 mutations are homozygous recessive. a) Which crosses would you set up to find out if the mutations are in the same gene or in different genes? b) You discover that turtle1 and turtle3 have mutations in the same gene, while turtle1 and turtle 2 have mutations in different genes. What phenotype would you expect from crosses between: Turtle3 x turtle2 Turtle1 x turtle3...
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