Lab 1 - Thin Layer Chromatography of Various Organic...

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Thin Layer Chromatography of Various Organic Compounds Experiment #1 Dan Guerra Section: 6 9/15/11
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Purpose: The purpose of this lab is to learn the technique of thin layer chromatography. The technique of thin layer chromatography is then used to differentiate various organic compounds by calculating and interpreting their R f values. Results and Discussion: In part A, the expectation was that the size of the TLC place would not affect the R f value for the compound of benzyl alcohol, as the same eluent was used for both plates. In part B, it was impossible to determine which eluent would have the highest attraction to the phenol solution spot until tested. In part C, the spot of benzoic acid was predicted to have the lowest mobile phase off the compounds tested, because of its polar –OH group, thus having a higher affinity to the stationary phase. In part D, it was difficult to predict precisely what F, D & C dyes would correspond to the commercial dyes, as the dyes were likely comprised of multiple dyes. After carrying out part A, the following data was recorded: Part A Distance traveled by solvent (cm) Distance traveled by compound (cm) R f value Small plate (4.70cm) 2.5 1.6 0.64 Large plate (6.90cm) 4.0 3.0 0.75 These results show a disparity between the R f values for the two plates. This however, should not be the case. By running both plates in the same eluent (eluent A) and spotting them with the same compound (benzyl alcohol), the resulting R f values should have been roughly the same. The difference in Rf values may be attributed to how evenly the solvent traveled up the TLC plates. However, the size of the TLC plate should have no
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effect on the R f value, as they are all covered in the same substance. After conducting part B, the following data was recorded:
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This note was uploaded on 02/15/2012 for the course ORGO 101 taught by Professor Staff during the Fall '11 term at Syracuse.

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Lab 1 - Thin Layer Chromatography of Various Organic...

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