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HOMEWORK 4 CELL ADHESION AND ECM KEY 2 revised 4/3 This figure shows cells from the developing liver and developing kidney of an embryonic mouse. The cells have each been dissociated in culture medium without Ca++. When they are mixed back together in medium with Ca++ they re-aggregate as shown. This figure applies to questions 1-4. 1. If the re-aggregation requires calcium, what class of cell-cell adhesion molecules is most likely to be causing re-aggregation? ANS 1: Cadherin (2pts) because cadherin’s require calcium to stick together (3pts) 2. Are the adhesion molecules on both the liver cells and the kidney cells likely to be identical, not just the same class of molecules? Explain your answer in a few sentences . ANS 2: Not identical because the cells sort from each other after readdition of calcium. If they were identical the cells would not sort from each other. 2PT for answer, 3PTS FOR EXPLANATION 3.Based on what you know about cell-cell adhesion, explain why the cells separate into two distinct clusters.
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