Lab 4 Bacterial Gene Transfer

Lab 4 Bacterial - Exercise 4 Bacterial Gene Transfer Zachary Bergman Friday PM Section 75 TA Rashmi 1 The purpose of the CSH121 and CSH125 controls

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Unformatted text preview: Exercise 4: Bacterial Gene Transfer Zachary Bergman Friday PM Section: 75 TA: Rashmi 1. The purpose of the CSH121 and CSH125 controls in part one is to confirm that we started with pure strains of the correct bacteria. Neither of the strains were supposed to grow until after they were mated and the controls in part one established that this was the case for our experiment. The purpose of the two leucine controls in part two is to confirm that the bacteria was actually plated on all seven dishes. If a colony grew on the first leucine plate, but not the second, then the bacteria must have all been removed from the toothpick before the final plate and therefore that specific colony is not reliable to the data for all the dishes. 2. It is important to obtain single colonies because we only want to test the genome of one progeny at a time. If two colonies are used per sample, it is possible that their genomes compliment each other, say one does not produce tyrosine and the other does allowing growth where there shouldn't be, giving...
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This note was uploaded on 02/16/2012 for the course BIOL_SCI 210-1 taught by Professor Mordacq during the Winter '10 term at Northwestern.

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Lab 4 Bacterial - Exercise 4 Bacterial Gene Transfer Zachary Bergman Friday PM Section 75 TA Rashmi 1 The purpose of the CSH121 and CSH125 controls

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