Lab 6 Complementation

Lab 6 Complementation - Exercise 6: Complementation Zachary...

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Exercise 6: Complementation Zachary Bergman Friday PM Section: 75 TA: Rashmi 1. The minimal medium with vitamins was used as a negative control because these plates contain none of the necessary amino acids required for growth, and the goal was to determine which of the crosses were able to produce their own adenine. The mutant colonies that were used in this experiment were lacking one of the genes necessary to complete the production of adenine, but the original plates all contained adenine to ensure that even the mutant colonies grew. By crossing both mutant and wild type colonies and then plating them on a minimal medium plate we were able to see which of the strains had genes that complimented each other in the adenine biosynthetic pathway. 2. The two strains must be deficient in different genes. This is due to the fact that the red a-mut1 and red ɑ -mut2 crossed to form a cream wild type strain which grew on the MV plate and the a- mut2 and ɑ -mut1 cross did as well. For two mutant strains to form wild type progeny, the
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Lab 6 Complementation - Exercise 6: Complementation Zachary...

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