Bio 210-2 Lab1 - Jason Kamin Bio 210-2 Lab#1 Enzymatic Analysis Introduction As the title of the lab suggests we were to analyze the effectiveness

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Jason Kamin Bio 210-2 1/31/08 Lab #1 Enzymatic Analysis Introduction As the title of the lab suggests, we were to analyze the effectiveness of the enzyme IDH under different circumstances such as concentration, pH, and cofactors. We were then to do a kinetics assay and inhibitor assay on IDH in order to develop Michaelis-Menten and Lineweaver-Burk plots for the enzyme. From these plots we would be able to determine the maximum velocity of the enzymatic reaction as well as Km of the reaction. This is all in an effort to understand the delicate balance that enzymes play in our lives and to what extent that they perform their functions. Materials and Methods Test tubes, with final volumes of 4 ml, were put into the spectrophotometer in order to get absorbance readings for each of the mixtures. In each of the test tubes were mixtures of assay buffer, NADP + , IDH and isocitrate; the mixtures varied based on concentration, pH and cofactors such as metal ions. When put into the spectrophotometer, readings
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This note was uploaded on 02/16/2012 for the course BIOL_SCI 210-2 taught by Professor Somebody during the Winter '09 term at Northwestern.

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Bio 210-2 Lab1 - Jason Kamin Bio 210-2 Lab#1 Enzymatic Analysis Introduction As the title of the lab suggests we were to analyze the effectiveness

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