Exam 3 2004 - Name _ Biological Sciences 210-2 Winter...

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Name _________________________ Biological Sciences 210-2 Winter Quarter 2003-2004 Third Exam Instructions: All exams must be done in black or blue ink. Any exam done in pencil will not be graded. Some exams will be photocopied before being returned to the students. Print your name on every page. Make sure the exam has 11 pages including the cover page. All answers must appear in the space provided. Use the backs of pages as scratch paper, but only answers appearing in the space provided will be graded. All exams must be turned in at the end of 2 hours. Page # 2_____ 7_____ 3_____ 8_____ 4_____ 9_____ 5_____ 10_____ 6_____ Total: _________/100 1
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Name _________________________ 1) All eukaryotic promoters for RNA polymerase II contain a TATA box. True / False (1pt) 2) Transcription from all eukaryotic promoters requires the protein TBP. True/False (1pt) 3) In class we discussed four different levels at which the concentration of proteins is regulated in eukaryotic cells. List all four of them. (4pts) 4) What is the relationship between DNA repair and the predisposition to cancer? (5pts) 5) A promoter relates to RNA synthesis as a/an _________________________________ relates to DNA replication. (2pts) 6) Several factors involved in translation in eukaryotic cells require GTP binding to be active. One of those factors is eIF2. What is the reason for the GTP requirement or for what purpose do cells use this requirement? (5 pts) 2
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Name _________________________ 7) DNA polymerases can synthesize DNA (1pt) a. de novo by catalyzing the polymerization of free dNTPs. b. by adding dNTPs to complementary dNTPs on a single-stranded DNA. c. by adding dNTPs to a hydroxyl group on the end of a growing polynucleotide chain hydrogen bonded to a strand of RNA. d. by adding dNTPs to a hydroxyl group on the end of a growing polynucleotide chain hydrogen bonded to a strand of DNA. 8) Short segments of newly synthesized DNA on the lagging strand of DNA are called (1pt) a. Okazaki fragments. b. replicons. c. origins of replication. d. lagging fragments. 9) Eukaryotic DNA polymerase β is active in non-dividing as well as dividing cells. This suggests that it (1pt) a. is the polymerase for leading strand replication. b. is the polymerase for lagging strand replication. c. fills in the gaps between Okazaki fragments. d. functions primarily in repair of DNA damage. 10) The short fragments of DNA produced during DNA replication are joined together by a. RNA polymerase. b. DNA polymerase. c. DNA helicase. d. DNA ligase. (1pt) 11) The function of topoisomerase II is to a. resolve DNA tangles. b. allow DNA to swivel and unwind. c. allow daughter chromatids to separate in anaphase. d. All of the above (1pt) 12) The fundamental property of DNA polymerase is that all a. synthesize DNA only in the 5 to 3 direction. b. can add deoxyribonucleatides only to a preformed primer strand.
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Exam 3 2004 - Name _ Biological Sciences 210-2 Winter...

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