Exam 3 2006 - NAME: _ 1) In class we discussed four...

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NAME: _______________________________ 1) In class we discussed four different levels at which the concentration of proteins is regulated in eukaryotic cells. List four of them. (4pts) 2) Name three mechanisms by which eukaryotic gene activation is facilitated. (3pts) 3) Name three features a plasmid vector should contain to clone a gene (3pts). 4) The first enzymatic step in making a cDNA uses which of the following? (2 pt) a. Eco RI nuclease b. Taq polymerase c. DNA polymerase d. RNA-dependent DNA polymerase 5) You are given the single stranded DNA template below, which is suitable for DNA sequencing. 5’-TTGCGTACGCTGCATATGGTCGTAGCGTATGCTGACTGACTGATCGCTC-3’ You are also given the following radioactively labeled primer: 3’-CATACGACTGACTG-5’ A) If you carried out sequences reaction in the presence of E. coli DNA polymerase and the four dNTPs, what would be the length of the radiolabeled product that is formed? (2pts) B) If you now carry out the same sequencing reaction (template, primer, dNTPs, polymerase), but include a small amount of dideoxy CTP in the mix, what would be the lengths of the radiolabeled products that would be formed? (2pts) 1
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NAME: _______________________________ 6) It is difficult to introduce DNA into plant cells because of the plant cell walls. Mention two methods that get around this problem. (2 pts) 7) Approximately 40% of the human genome are simple sequence DNA. For much of this DNA its function is not known. However, simple sequence DNA is used in biotechnology applications. Name an example. (2pt) 8) The human genome is approximately 1000 times larger than the genome of E. coli. However, the human genome contains only approximately 10 times more genes than the E. coli genome. Name two reasons? (3pts) 9) The figure shows a two-locus DNA fingerprinting gel using DNAs from a crime victim (V), blood taken from the scene of the crime (E), and two suspects (S1, S2, and S3). Can you rule out any of the suspects, and if so, which one? Based on the evidence, could you convict any of these suspects beyond a reasonable doubt? If you are unable to draw firm conclusions state what other information you would require for a firm conclusion. (3pts) 2 V E S1 S2 S3
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NAME: _______________________________ 10) The consensus sequence of the E. coli -10 promoter element it TATAAT. You are comparing two promoters, one of which as a -10 element sequences of TATGAT and the other a -10 element sequence of CATGAT. Can you make any predictions about how well the two promoters will support the initiation of transcription? If yes, which is the stronger promoter and why? (3pts) 11)In class we talked about how bacterial cells can take up the amino acid tryptophan from their surroundings, or if the external supply is insufficient, they can synthesize trytophan by using enzymes in the cell. In some bacteria, the control of glutamine synthesis is similar to that of tryptophan synthesis, such that the glutamine repressor is used to inhibit the transcription of the
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Exam 3 2006 - NAME: _ 1) In class we discussed four...

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