Lab 1 PAGE of Blood Proteins

Lab 1 PAGE of Blood Proteins - Lab 1 Polyacrylamide Gel...

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Lab 1: Polyacrylamide Gel Electrophoresis (PAGE) of Blood Proteins Zachary Bergman Partner: Michael Roche TA: Sujit Jangam Bio 210-3 Introduction The goal of this lab was to run a Polyacrylamide Gel Electrophoresis (PAGE) on the proteins present in bovine blood cells and plasma. The samples that are run in the PAGE will undergo a variety of different protein separation techniques to determine the effectiveness of each. The techniques include centrifugation to separate the proteins based on size, the addition of ammonium sulfate to separate based on solubility in water, glycosylation and phosphorylation. The PAGE results will tell us the likely function and association of the proteins that are present in the bovine blood. Results G CY+ CY- AS+ AS- AP+ AP- 340 (0.3 cm) 32 (9.2 cm) 29 (10.3 cm) 160 (1.3 cm) 120 (2.0 cm) 340 (0.3 cm) 300 (0.4 cm) 220 (0.8 cm) 12 (17.6 cm) 12 (17.4 cm) 64 (4.0 cm) 55 (5.0 cm) 200 (0.9 cm) 200 (0.9 cm) 120 (1.8 cm) 25 (10.9 cm) 25 (10.9 cm) 125 (1.9 cm) 125 (1.9 cm) 81 (3.2 cm) 100 (2.6 cm) 51 (5.5 cm) 47 (6.2 cm) 53 (5.3 cm) 28 (10.4 cm) 38 (7.7 cm) 29 (10.2 cm) 23 (12.0 cm) 32 (9.2 cm) 24 (11.6 cm) 12 (17.3 cm) Table 1. Molecular Weight of Proteins in kD (Distance Traveled in cm) Also, please see attached data tables and graphs.
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