Lab 3 Signal Tranduction in Yeast

Lab 3 Signal Tranduction in Yeast - Lab 3: Signal...

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Lab 3: Signal Transduction in Yeast Zachary Bergman Partner: Michael Roche TA: Sujit Jangam Bio 210-3 Introduction The goal of this lab is lab is to study the signal transduction pathways in the yeast Saccharomyces cerevisia. For the experiment we used the wildtype and two mutant strains which will be called Mutant X and Mutant Y. The mutated gene is called FUS1 which codes for enzyme β- galactosidase when activated by an α factor. The production of this enzyme effects the mating of the yeast and this is how we will determine the effects of each mutation. The compound O- nitropheylgalactophyranoside (ONPG) can also be used to measure the β-galactosidase concentration by cleaving it into o-nitrophenyl and galactose. O-nitrophenyl is yellow and therefore its concentration can be measured using a spectrophotometer. Results β-galactosidase Activity Yeast Culture Activity (Miller Units) WT 15.9 WT + α Factor 914.9 Mutant X 14.3 Mutant X + α Factor 764.9 Mutant Y 3.02 Mutant Y + α Factor 5.17 Table 1. Activity of different yeast strains with and without α Factor
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This note was uploaded on 02/16/2012 for the course BIOL_SCI 210-3 taught by Professor Somebody during the Spring '09 term at Northwestern.

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Lab 3 Signal Tranduction in Yeast - Lab 3: Signal...

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